SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Qin,Wei; Fan,Fuqiang; Zhu,Yi; Huang,Xiaolong; Ding,Aizhong; Liu,Xiang; Dou,Junfeng.

Abstract Cellulosimicrobium cellulans CWS2, a novel strain capable of utilizing benzo(a)pyrene (BaP) as the sole carbon and energy source under nitrate-reducing conditions, was isolated from PAH-contaminated soil. Temperature and pH significantly affected BaP biodegradation, and the strain exhibited enhanced biodegradation ability at temperatures above 30 °C and between pH 7 and 10. The highest BaP removal rate (78.8%) was observed in 13 days when the initial BaP concentration was 10 mg/L, and the strain degraded BaP at constant rate even at a higher concentration (50 mg/L). Metal exposure experimental results illustrated that Cd(II) was the only metal ion that significantly inhibited biodegradation of BaP. The addition of 0.5 and 1.0 g/L glucose enhanced...

Analysis of methane biodegradation by Methylosinus trichosporium OB3b

Palavras-chave: Anaerobic; Biodegradation; Benzo(a)pyrene; Cellulosimicrobium cellulans CWS2.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200258

Rodrigues,Andréa dos Santos; Valdman,Belkis; Salgado,Andréa Medeiros.

The microbial oxidation of methane in the atmosphere is performed by methanotrophic bacteria that use methane as a unique source of carbon and energy. The objective of this work consisted of the investigation of the best conditions of methane biodegradation by methanotrophic bacteria Methylosinus trichosporium OB3b that oxidize it to carbon dioxide, and the use of these microorganisms in monitoring methods for methane. The results showed that M. trichosporium OB3b was capable to degrade methane in a more effective way with an initial microorganism concentration of 0.0700 g.L-1, temperature of 30ºC, pH 6.5 and using 1.79 mmol of methane. In these same conditions, there was no bacterial growth when 2.69 mmol of methane was used. The specific rate of...

Anti-Fusarium moniliforme activity and fumonisin biodegradation by corn and silage microflora

Palavras-chave: Methane; Biodegradation; Methanotrophic bacteria; Methylosinus trichosporium OB3b.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200017

Camilo,Simone B.; Ono,Carlos José; Ueno,Yoshio; Hirooka,Elisa Y..

Studies were carried out to isolate microorganisms from corn and silage screened for their ability to inhibit F. moniliforme growth (strain 113F) in association with fumonisin detoxification. Among 150 isolates four Gram-positive bacilli and one yeast with inhibitory activity were selected. The inhibition zone ranged from 50 to 72.5 mm using cultures, and from 25 to 52.5mm for crude alcoholic extract. The isolates S9, S10, S69 (sporulated bacilli) and SE3071 (yeast) degraded 43, 48, 83 and 57% of the initial FB1 concentration, respectively. The pH increased gradually in the medium during incubation for biodegradation process.

Application of molecular fingerprinting for analysis of a PAH-contaminated soil microbiota growing in the presence of complex PAHs - DOI: 10.4025/actascibiolsci.v32i1.7575

Palavras-chave: Fusarium moniliforme; Fumonisin; Biodegradation.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000200004

Biological Sciences

Silva, Ísis Serrano; UNICAMP; Menezes, Cristiano Ragagnin de; UNICAMP; Faria, Andréia Fonseca de; UNICAMP; Costa, Josemar Gurgel da; UFRN; Pepper, Susan; The University of Melbourne; Britz, Margaret; The University of Melbourne; Durrant, Lucia Regina; UNICAMP.

Polycyclic aromatic hydrocarbons (PAHs) constitute a group of priority pollutants which are present at high concentrations in the soils of many industrial contaminated sites. Pollution by these compounds may stimulate growth of organisms able to live in these environments causing changes in the structure of the microbial community due to some cooperative process of metabolization of toxic compounds. A long-term PAH-contaminated soil was stored for several years and used to analyze the native microbiota regarding their ability to grow on pyrene, benzo[a]pyrene, as well as in mixtures of LMW- and HMW-PAHs. Molecular profiles of the microbial community was assessed by PCR-DGGE of 16S rRNA gene, and the number of bands observed in DGGE analyses was interpreted...

Aspects ultrastructuraux de la biodegradation de l''ancienne cuticule de Carcinus maenas durant la periode preecdysiale

Palavras-chave: Polycyclic aromatic hydrocarbons (PAHs); Biodegradation; PCR-DGGE; Microbial community Polycyclic aromatic hydrocarbons (PAHs); Biodegradation; PCR-DGGE; Microbial community.

Ano: 2009

URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7575

ArchiMer

Thorez, A; Compere, P; Goffinet, G.

Ultrastructural observations of various integumental regions of the crab Carcinus maenas show an important biodegradation of the old sclerites during premolt. At the ecdysis, this biodegradation extends from the basis of the membranous layer up the upper third of the principal layer. The first biodegradation features are a disorganization followed by a complete disappearance of the microfibrils. Numerous ecdysial droplets and double membrane bounded microstructures probably take part in biodegradation processes.

Tipo: Text

Palavras-chave: Carcinus maenas; Malacostraca; Ultrastructure; Moulting; Biodegradation.

Ano: 1987

URL: http://archimer.ifremer.fr/doc/1987/acte-1376.pdf

Azo Dye Mineralization by Phanerochaete Chysosporium in a Sequencing Bath Reactor

Wanderley,Carlos Ronald Pessoa; Andrade,Marcus Vinícius; Pereira,Luciana José; Silva,Gloria Maria Marinho; Pessoa,Kelly Rodrigues.

ABSTRACT The mineralization of the azo dye congo red by the fungi Phanerochaete chrysosporium was studied in two sequential batch bioreactors (R1 and R2), operated in cycles of 48 h (step I) and 24 h (step II). In step I, glucose concentration was 1 g.L-1 in both reactors and in step II, 1 g.L-1 of glucose was maintained in R1, but R2 received no addition of glucose. In step I, the average dye removal efficiencies were 76 ± 29 % (R1) and 53 ± 15% (R2), while in step II the averages recorded for dye removal for R1 and R2 were 84 ± 15 and 70 ± 28%, respectively. The rates of dye removal were 0.04 h-1 in R1 and 0.03 h-1 in R2 in step I. Higher rates were obtained in step II, 0,07 h-1 and 0,02 h-1 for R1 and R2, respectively. The highest dye removal occurred...

Banco de Extratos de Enzimas Fibrolíticas Isoladas de Conteúdo Ruminal (BEEFRUM).

Palavras-chave: Biodegradation; Congo red; Phanerochaete chrysosporium; Sequential bioreactors.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100606

Infoteca-e

SALLES, H. O.; EGITO, A. S. do; CARNEIRO, J. da C.; XIMENES, L. V.; RIBEIRO, M. T.; RIBEIRO, R. P.; BARBOSA, I. C.; SOUSA, A. M. P..

A criação do banco de extratos composto por enzimas fibrolíticas isoladas de conteúdo ruminal de bovinos, caprinos e ovinos, foi iniciativa da Embrapa Caprinos e Ovinos e da Embrapa Gado de Leite, através de projetos de pesquisa em parceria, e tem como objetivo salvaguardar extratos enzimáticos de nossa biodiversidade para utilização biotecnológica, visando caracterizar, avaliar e identificar possíveis aplicabilidades científicas e/ou industriais das enzimas contidas nesses extratos.

Tipo: Comunicado Técnico (INFOTECA-E)

Palavras-chave: Bioetanol; Enzimas fibrolíticas; Sugar byproducts.; Cellulolysis; Etanol; Cana de açúcar; Bagaço; Celulase; Enzima celulolítica; Cellulose; Biodegradation; Sugarcane; Ethanol..

Ano: 2017

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1087003

Benzene, toluene and xylene biodegradation by Pseudomonas putida CCMI 852

Otenio,Marcelo Henrique; Silva,Maria Teresa Lopes da; Marques,Maria Luiza Oliveira; Roseiro,José Carlos; Bidoia,Ederio Dino.

A minimal liquid medium containing benzene (B), toluene (T) and xylene (X) and mixtures thereof, was used to evaluate degradation activity of Pseudomonas putida CCMI 852 containing a TOL plasmid. Experiments were developed with B, T and X (100 mg L-1), with mixtures of BT, BX, and TX (50 + 50 mg L-1 each) and BTX (33.3 + 33.3 + 33.3 mg L-1 each), added to 500 mL of medium. After 18 to 24 hours, the inoculum was added and solvent disappearance was determined after 24 to 25 hours by GC. Results showed that P. putida CCMI 852 was able to metabolize T and X, but B was not metabolized. In a BTX mixture, B was not metabolized and T and X degradation rate decreased 50%.

Best conditions for biodegradation of diesel oil by chemometric tools

Palavras-chave: Pseudomonas putida; Biodegradation; BTX; Plasmid TOL.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300010

Kaczorek,Ewa; Bielicka-Daszkiewicz,Katarzyna; Héberger,Károly; Kemény,Sándor; Olszanowski,Andrzej; Voelkel,Adam.

Diesel oil biodegradation by different bacteria-yeast-rhamnolipids consortia was tested. Chromatographic analysis of post-biodegradation residue was completed with chemometric tools (ANOVA, and a novel ranking procedure based on the sum of ranking differences). These tools were used in the selection of the most effective systems. The best results of aliphatic fractions of diesel oil biodegradation were observed for a yeast consortia with Aeromonas hydrophila KR4. For these systems the positive effect of rhamnolipids on hydrocarbon biodegradation was observed. However, rhamnolipids addition did not always have a positive influence on the biodegradation process (e.g. in case of yeast consortia with Stenotrophomonas maltophila KR7). Moreover, particular...

Biocompatibility, osteointegration, osteoconduction, and biodegradation of a hydroxyapatite-polyhydroxybutyrate composite

Palavras-chave: Biodegradation; Chemometrics; Systems ranking; Variance analysis.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100017

Reis,Emily Correna Carlo; Borges,Andréa Pacheco Batista; Fonseca,Cláudio César; Martinez,Mastoby Miguel Martinez; Eleotério,Renato Barros; Morato,Gláucia Oliveira; Oliveira,Paulo Miranda.

In this work, biocompatibility, osteointegration, osteoconductivity, and biodegradation of a hydroxyapatite polyhydroxybutyrate new composite were evaluated. The composite was implanted in rabbits' bone defects and clinical, radiographic, histological, and histomorphometric data of these animals were compared with those of unfilled defects on the days 8th, 45th, and 90th after surgery. No significant differences existed between the groups for the evaluated clinical parameters. Radiographs showed bone-composite direct contact. Bone formed within the defect, interface and inside the composite. Significant differences were found between the bone and connective tissues percentage within the defect at all dates and at the interface on the 45th day, bone tissue...

Biodegradation and toxicological evaluation of lubricant oils

Palavras-chave: Biomaterials; Bone; Biocompatibility; Biodegradation; Hydroxyapatite-polyhydroxybutyrate composite.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400010

Tamada,Ivo Shodji; Lopes,Paulo Renato Matos; Montagnolli,Renato Nallin; Bidoia,Ederio Dino.

The aim of this work was to compare different toxicity levels of lubricant oils. The tests were performed using the earthworm (Eisenia andrei), arugula seeds (Eruca sativa) and lettuce seeds (Lactuca sativa), with three types of contaminants (mineral lubricant oil, synthetic lubricant oil and used lubricant oil) for various biodegradation periods in the soil. The toxicity tests indirectly measured the biodegradation of the contaminants. The samples were analyzed at t0, t60, t120 and t180 days of biodegradation. The used lubricant oil was proved very toxic in all the tests and even after biodegradation its toxicity was high. The mineral and synthetic oils were biodegraded efficiently in the soil although their toxicity did not disappear completely after 180...

Biodegradation du petrole en mer. Influence de l'apport d'azote et de phosphore sous forme minerale

Palavras-chave: Toxicology; Biodegradation; Lubricant oil; Toxicity bioassay.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600020

ArchiMer

Lepetit, J; Guiot, J; Martin, Y; Tagger, S.

To exploit the presence in the sea of microorganisms capable of degrading petroleum products a mineral fertilizer, containing immediatly assimilable nitrogen and phosphorus compounds, was used to increase the microbial population and therefore, the degradation potential of treated polluted waters. The fertilizer supply caused an increase in heterotrophic and hydrocarbon-splitting bacteria and an increase in the phytoplanktonic biomass. The latter is characterized by an increase in a few selected species; it constitutes an important supply of organic substances, and it is responsible for increasing the dissolved oxygen which is essential for petroleum oxidation. Supplying the fertilizer results in an increase in the treated water degradation potential and a...

Tipo: Text

Palavras-chave: Bacteria; Microorganisms; Heterotrophic organisms; Phosphorus compounds; Nitrogen compounds; Fertilizers; Hydrocarbons; Biodegradation; Sea water.

Ano: 1984

URL: http://archimer.ifremer.fr/doc/1984/acte-1020.pdf

Biodegradation kinetics of bromoxynil as a pollution control technology

OceanDocs

Askar, A.I.; Ibrahim, G.H.; Osman, K.A..

Nonpoint source (NPS) pollution from agriculture is the leading source of impairment to Survey Rivers and lakes. Pesticides are one of the major NPS pollutants that result from agricultural activities. Among those pesticides, is Bromoxynil (BRMX) which is a widely used herbicide. The present study was carried out to determine the capability of selected biological control agents to degrade BRMX at different incubation periods. Microbial degradation of BRMX at the rate of 100 ppm in pure liquid culture media of either seven isolates of bacteria; Azotobacter chroococcum, Azospirillium barasilense, Klebsilense pneumoneae, Pseudomonas cepacia, Pseudomonas fluoresences, Bacillus subtilis, and Bacillus polymyxa or two isolates of fungi; Trichoderma viride and...

Tipo: Journal Contribution

Palavras-chave: Laboratory Study; Biodegradation; Pollution; Biodegradation; Pollution; Http://aims.fao.org/aos/agrovoc/c_9261; Http://aims.fao.org/aos/agrovoc/c_6077.

Ano: 2007

URL: http://hdl.handle.net/1834/2200

Biodegradation kinetics of o-cresol by Pseudomonas putida DSM 548 (pJP4) and o-cresol removal in a batch-recirculation bioreactor system

Electron. J. Biotechnol.

Kaymaz,Yasin; Babaoglu,Anil; Pazarlioglu,Nurdan Kasikara.

The biodegradation kinetics of o-cresol was examined by acclimatized P. putida DSM 548 (pJP4) in batch experiments at varying initial o-cresol concentrations (from 50 to 500 mg/L). The kinetic parameters of o-cresol aerobic biodegradation were estimated by using the Haldane substrate inhibition equation. The biodegradation kinetics of o-cresol was investigated. In batch culture reactors, the Maximum specific growth rate (μmax), Monod constant (Ks) and the inhibition constant (Ki) were established as 0.519 h-1, 223.84 mg/L and 130.883 mg/L, respectively. o-cresol biodegradation in a batch-recirculation bioreactor system by immobilized P. putida was also studied. The recycled packed bed reactor system, which was composed of Ca-alginate beads and...

Tipo: Journal article

Palavras-chave: Biodegradation; Cell immobilization; O-cresol; Pseudomonas putida; Substrate inhibition kinetics.

Ano: 2012

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100003

Biodegradation of a blended starch/natural rubber foam biopolymer and rubber gloves by Streptomyces coelicolor CH13

Electron. J. Biotechnol.

Watcharakul,Sirimaporn; Umsakul,Kamontam; Hodgson,Brian; Chumeka,Wannapa; Tanrattanakul,Varaporn.

Background: The growing problem of environmental pollution caused by synthetic plastics has led to the search for alternative materials such as biodegradable plastics. Of the biopolymers presently under development, starch/natural rubber is one promising alternative. Several species of bacteria and fungi are capable of degrading natural rubber and many can degrade starch. Results: Streptomyces coelicolor CH13 was isolated from soil according to its ability to produce translucent halos on a mineral salts medium, MSM, supplemented with natural rubber and to degrade starch. Scanning electron microscope studies showed that it colonized the surfaces of strips of a new starch/natural rubber biopolymer and rubber gloves and caused degradation by forming holes,...

Tipo: Journal article

Palavras-chave: Biodegradation; Biopolymer; Natural rubber; Starch; Streptomyces coelicolor.

Ano: 2012

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100008

Biodegradation of acetonitrile by cells of Candida guilliermondii UFMG-Y65 immobilized in alginate, kappa-carrageenan and citric pectin

Dias,João Carlos T.; Rezende,Rachel P.; Linardi,Valter R..

Different encapsulation matrices were tested for immobilized cells of Candida guilliermondii UFMG-Y65 used for acetonitrile degradation. Acetonitrile degradation by free cells and cells immobilized in Ba-alginate, kappa-carrageenan and citric pectin was studied. The rate of acetonitrile degradation was monitored for 120 h by measuring yeast growth and ammonia concentration. Different alginate concentrations did not affect cell viability, but the period of incubation in BaCl2 solution reduced the number of viable cells. Likewise, the gel nature and the matrix structure of the support resulting from the cell immobilization conditions were of fundamental importance for biocatalyst activity and performance, affecting substantially the patterns of microbial...

Biodegradation of crude petroleum oil and environmental pollutants by Candida tropicalis strain

Palavras-chave: Candida guilliermondii; Acetonitrile; Biodegradation; Immobilization.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100015

Farag,Soha; Soliman,Nadia A..

A local yeast isolate named A was isolated from polluted area of Abou-Qir gulf (Alexandria, Egypt), identified according to a partial sequence of 18sRNA as Candida tropicalis. The isolate showed a high potency in petroleum oil biodegradation as well some hydrocarbons. Morphological changes in cell diameter of this yeast were recognized upon growing the target cell in sea water medium supplemented with petroleum oil as sole carbon source in comparison to the growth in enriched medium. Statistically-based experimental design was applied to evaluate the significance of factors on petroleum oil biodegradation by this yeast isolate. Eleven culture conditions were examined by implementing Plackett-Burman factorial design where aeration, NH4Cl and K2HPO4 had the...

Biodegradation of deproteinized potato wastewater and glycerol during cultivation of Rhodotorula glutinis yeast

Palavras-chave: Petroleum oil; Candida tropicalis; 18Sr RNA; Biodegradation; Plackett-Burman.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400023

Electron. J. Biotechnol.

Kot,Anna Maria; Blazejak,Stanislaw; Kurcz,Agnieszka; Gientka,Iwona.

Background Deproteinized potato wastewater and glycerol are two by-products which are difficult to dispose. The objective of this study was to determine the ability of Rhodotorula glutinis to use glycerol and nitrogen compounds present in deproteinized potato wastewater and to evaluate the ability of simultaneous biodegradation of potato wastewater and glycerol via microbiological methods. Results It has been found that R. glutinis used glycerol and potato wastewater as a source of carbon and nitrogen, respectively. The highest degree of glycerol content (70.6%) reduction was found after cultivation of the investigated strain using a medium with 5% glycerol. In this medium, a significant reduction in the total protein content, estimated at 61%, was...

Tipo: Journal article

Palavras-chave: Biodegradation; Chemical oxygen demand; Industrial waste; Rhodotorula.

Ano: 2015

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600007

Biodegradation of diesel oil by yeasts isolated from the vicinity of suape port in the state of Pernambuco - Brazil

Miranda,Rita de Cássia; Souza,Cynthia Silva de; Gomes,Edelvio de Barros; Lovaglio,Roberta Barros; Lopes,Carlos Edison; Sousa,Maria de Fátima Vieira de Queiroz.

The aim of this work was to investigate the potential of the diesel oil degrading yeasts to use them in bioremediation of areas contaminated by this pollutant. The cultures, identified as Rhodotorula aurantiaca UFPEDA 845 and Candida ernobii UFPEDA 862, were selected at the initial stage. In the course of the biodegradation assays, C. ernobii degraded tetradecane, 5 methyl-octane and octadecane completely and decane (60.8%) and nonane (21.4%) partially whilst R. aurantiaca presented degradation percentages of 93% for decane, 38.4% for nonane and 22.9% for dodecane.

Biodegradation of [D-Leu1] microcystin-LR by a bacterium isolated from sediment of Patos Lagoon estuary, Brazil

Palavras-chave: Biodegradation; Yeasts; Diesel oil.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100018

J. Venom. Anim. Toxins incl. Trop. Dis.

Lemes,Gilmar AF; Kist,Luiza W; Bogo,Mauricio R; Yunes,João S.

Background Toxic cyanobacterial blooms are recurrent in Patos Lagoon, in southern Brazil. Among cyanotoxins, [D-Leu1] microcystin-LR is the predominant variant whose natural cycle involves water and sediment compartments. This study aimed to identify and isolate from sediment a bacterial strain capable of growing on [D-Leu1] microcystin-LR. Sediment and water samples were collected at two distinct aquatic spots: close to the Oceanographic Museum (P1), in Rio Grande City, and on São Lourenço Beach (P2), in São Lourenço do Sul City, southern Brazil. Methods [D-Leu1] microcystin-LR was isolated and purified from batch cultures of Microcystis aeruginosastrain RST9501. Samples of water and sediment from Rio Grande and São Lourenço do Sul were collected....